PhD student in Deuchars lab: Oct 2015 – Oct 2019 co-supervised by Dr Julie Aspden
2015-present PhD in Biomedical Sciences, University of Leeds ‘Exploring the role of the molecular clock in sympathetic preganglionic neurons’ supervised by Prof Jim Deuchars, Dr Julie Aspden and Dr. Sue Deuchars
2007-2011 BSc Biology and Biochemistry, University of Winnipeg
Circadian rhythm of gene expression, neuroanatomy, systems neuroscience.
Christian is a PhD student studying the role of the molecular clock in sympathetic preganglionic neurons and diurnal rhythm of gene expression in these cell types using immunohistochemistry, qPCR and single cell RNA-seq.
Skills and techniques
•Fresh fixation preparation of tissue for RNA extraction, kit-based and phenol-chloroform RNA extraction, reverse transcription, primer design, quantitative polymerase chain reaction, agarose gel electrophoresis. Kit-based RNA extraction from single cell or low RNA input, bead-based cDNA purification. •Home Office license obtained. In vivo work includes tracing studies, perfusion fixation, and perfusion for slices (mice). Preparation of tissue via vibrating microtome and cryostat, immunohistochemistry, confocal microscopy. •Received training in R software, MobaXterm remote computing software for processing of next generation sequencing data. Raven analysis software, iWorx physiology software and Neurons in Action software, SPSS statistics software (previous demonstrating experience and projects).